Microfluidics and Microtiter Plates

    The fluid wall platform is an excellent way to build a microfluidics chamber. The platform is a convenient, automated method that uses interfacial forces to build a wall. It can be built quickly and efficiently, from a 6-cm dish to a full plate. Furthermore, the system can be scaled up to larger sizes. The array in Figure 1 C has the equivalent density of 393,216 microplates.

    The microtiter plate is an array of miniature test tubes that have uniform footprints. The working volumes range from 15 to 150 mL, three to 10 mL, and 100 mL. Various arrays have been developed, ranging from a few hundred to thousands of wells. Recently, arrays with volumes down to femtoliters have been made. Another variant of the chamber is a collection of aqueous drops sitting on a flat surface.

    The microfluidic chamber is a chip made of two-dimensional hydrodynamic flow-through traps. The height of the microfluidic chamber is 45 um. The height of the microfluidics chamber is chosen based on the protoplast size distribution and the dimensions of the extracellular electrodes. This enables the traps to capture the desired volumes while maximizing mechanical stability and immobilization. At the same time, the size of the traps is chosen carefully to avoid strong confinement, which may alter the development of the protoplasts. Go to website to learn more about microfluids.

    The main challenge with using microfluidics chambers in microfluidics is the fact that the fluid does not have equal height across the dish. The solution to this problem is shown in Fig. 2 E, where three six-cm dishes are covered with FC40 and blue dye. Then, the dishes are marked by lines, creating three large central chambers. Different dye volumes are added to the chamber.

    A three-chamber compartmentalized microfluidic device enables manipulation of synapses. The device has a perfusion channel and integrated local perfusion chamber. The enlarged image shows the direction of the fluid flow. In addition, a merged fluorescence and DIC image shows the perfusion of the dye Alexa Fluor 488. The third image, which is an ocular imaging of the nanofluidics chamber, reveals the perfusion of the laminin on the neuron.

    The microfluidic chamber is a versatile device used to study cell migration and neuronal processes. The technology is based on a replica mold process and uses a soft lithography process. It is inexpensive and can be fabricated in biological laboratories without clean-room facilities. The chemotaxis microchannel barrier can isolate neutrophils from neuronal bodies, while the multicompartment culture chambers isolate different types of cells, including the cell body and the neuronal processes. Click here for more information about the microfluidics chamber.

    The microfluidics chamber is a versatile tool for studying cells. Researchers use it to measure individual cells in worms. The chamber can be used to screen candidate molecules for axonal regeneration. One study, conducted by Taylor and colleagues, uses a microfluidics chamber to detect RNA expression in neurons. There are many uses for a cell-based bioassay. In addition to analyzing the function of cells, it can be used to screen for molecules that can influence their migration patterns. Find out more details in relation to this topic here: https://en.wikipedia.org/wiki/Microfluidics.


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